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Figure 7.
FIG. 7. Crystal structure of the thermostable mutants of
CjXyn10A. Panel a shows orthogonal views of CjXyn10A with the
three sites of improved stability indicated; the calcium binding
loop, Ala-80, and the N- and C-terminal "contact" region where
an inserted S-S bridge contributes to stability. Panel b reveals
the structural basis of the A80T mutation. The introduced
threonine both fills a void and, thus, makes van der Waals
contacts with surrounding residues and allows a favorable
hydrogen-bond (dotted yellow line) with Asp-123. This figure was
drawn with PyMol (DeLano Scientific, San Carlos, CA) and is in
divergent ("wall-eyed") stereo.
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