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Figure 7.
Figure 7. Domain Twisting Caused by ATP Binding to the Open
Ring of GroEL-GroES-ADPCryo-EM structure to 12.5 Å
resolution of the GroES-ADP[7]-GroEL-ATP[7] complex isolated
from a reaction mixture. The cryo-EM map is shown as a blue
transparent surface, with the atomic structure docked in. The
GroEL domains are colored as in Figure 2, and GroES is cyan. The
precision of the fit is demonstrated by the side view (a). The
white circle in (a) indicates the position of the R197-E386
contact. The free apical domains (lower ring in [a]) are docked
into the end view (b) with a counterclockwise rotation of
20° ± 5°. The white dotted lines are orientation
markers for comparison of the apical rotation here with that in
Figure 3a.
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