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Figure 7.
Fig. 7. Overlay of TLM and cerulenin in the FabB active
site. The FabB-TLM and FabB-cerulenin structures were
superimposed to illustrate the differences in the binding of the
antibiotics in the active site. The coloring scheme in this
figure is the same as in Figs. 3 and 4. TLM binds on the
malonyl-ACP side and cerulenin occupies the acyl-enzyme
intermediate half. The O-1 of TLM and O-2 of cerulenin are the
only portions of the antibiotics that overlap in the structure,
and they form hydrogen bonds with the His-His dyad in the active
site. Note that the protein structure shown is that of the
FabB-TLM complex. Binding of the two antibiotics results in
essentially identical changes in the conformations of the active
site residues.
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