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Figure 6.
FIGURE 6. A, surface representation of PezT alone, colored
according to Fig. 1A. Residues that are identical with zeta and
showed to impair PezT toxicity are colored in blue. An ATP
molecule colored in magenta was modeled according to the
position found in the chloramphenicol-phosphotransferase (29).
B, effect of PezT mutations on the growth of E. coli
BL21(DE3)/pLysS carrying recombinant pET11a plasmids as
determined by A[600] and log CFU. Growth profile of wild-type
PezT is shown as (x) for A[600] values (on top) and black bars
in log CFU (bottom). Growth profiles of the following mutants
following IPTG induction are shown: T117V (  ; green bars), T120V (
 ;
blue bars), and K45A (  ; yellow bars). The
R157A, R170A, and D66T mutations yielded nontoxic growth
profiles similar to those of K45A and T117V and are not
presented in the figure.
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