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Figure 6.
FIGURE 6. A conserved conformation of eEF2 and EF-G. A,
fusidic acid related mutations mapped on the crystal structure
of EF-G (Protein Data Bank entry 1FNM). The backbone of 30
fusidic acid resistance residues (41) is colored blue. B,
mapping onto an EF-G model derived from the conformation of eEF2
observed by cryo-EM (see text for details). The closer approach
of domain III to domain I in this model is illustrated by the
distances between Leu^457 and Gln^117 (dashed lines). The
distance is 16.5 Å in the crystal structure and 12.1
Å in the modeled structure. C, overlay of domains III
(blue), IV (red), and V (green) of eEF2-sordarin and the
equivalent domains of the T. thermophilus EF-G homolog (all
domains gray) from Protein Data Bank entry 1WDT illustrating a
highly conserved domain arrangement.
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