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Figure 6.
FIGURE 6. Functional activity of PikC mutants. High
pressure liquid chromatography analyses of PikC-catalyzed
reactions using YC-17 (A series) and narbomycin (B series) as
substrate are shown. A1/B1, negative control in the absence of
PikC. A2/B2, PikC wild type (PikC-wt). Mutants are used as
indicated in the figure. Compound identities are as follows: 1,
YC-17; 2, neomethymycin; 3, methymycin; 4, narbomycin; 5,
pikromycin. Conversion of narbomycin at low efficiency is
probably due to use of exogenous redox partners (e.g. spinach
ferredoxin reductase) in P450 reconstitution assays.
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