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Figure 6.
Figure 6. Two conformations of the E151D monomer in stereo.
The E151D monomer has an N-terminal dsDNA-binding domain (top)
and a larger ATPase core domain (bottom). The ATPase domain has
two half-sites for binding ATP (gold). Two putative DNA-binding
regions L1 (green) and L2 (magenta and blue for the two observed
conformations, respectively) are located near the filament axis
(dotted vertical line). Both ends of the L2 region contact the
ATP-binding site. Two K^+ (in yellow) were observed to form
bridges between the γ–phosphate of AMP–PNP and the short
helix in the L2 region (in magenta). When KCl was substituted
for NaCl, the L2 region (in blue) became largely disordered.
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