Figure 6.
Figure 6 The active sites of SfiI (left) and BglI (right). The
DNA and the active site residues of SfiI (Asp 79, Asp100, and
Lys102) and BglI (Asp116, Asp142, and Lys144) are shown in
'stick' representation. The scissile phosphodiester is indicated
by arrows. The Ca^2+ ions are shown as nonbonded spheres and
colored aqua, water molecules are not shown. In SfiI, the second
Ca^2+ is missing and the scissile phosphodiester is 3
Å further away than in BglI, indicating that the enzyme is in an
inactive state.
The above figure is reprinted
by permission from Macmillan Publishers Ltd:
EMBO J
(2005,
24,
4198-4208)
copyright 2005.
3
Å further away than in BglI, indicating that the enzyme is in an
inactive state.