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Figure 6.
Figure 6. Formation of the Annexin I Binding Surface from
Calcium Binding to Apo-S100A11Accessible surface area
representations of apo-S100A11 (A) and calcium-bound S100A11 (B)
in complex with annexin I (Rety et al., 2000). Each protein is
oriented approximately 90° with respect to that shown in Figure
4. In both molecules, residues in S100A11 that directly interact
(<5 Å) with annexin I are indicated and colored dark blue and
those whose side chain exposure increases by >20% upon calcium
binding but have less interaction are colored cyan. For
apo-S100A11 (A), many of these residues have less than 20% of
their side chains accessible to the surface and are disjoint on
the surface of the protein. In the calcium-bound structure (B),
most residues that contact the annexin I peptide increase their
side chain-accessible surface areas by >20% as shown in Figure
5C to form a contiguous site for the annexin interaction (dark
blue). To facilitate comparison, all sequence numbers correspond
to those of rabbit S100A11.
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