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Figure 6.
Figure 6. Yeast bypass results of mutations of MCM5 at Pro83
supporting the domain-push hypothesis for bob1 bypass mechanism.
a, Cells of S. cerevisiae strain 302 with cdc7ts containing
either wild type or mutant alleles of MCM5 were diluted serially
from 10^0 to 10^-4 (right to left), placed on YPD plates (see
Methods) and then incubated at either permissive (22 °C) or
non-permissive (36 °C) temperature for 2 d. b, Summary of
results. Yes indicates growth and No means no growth at the
indicated temperature. *Bypass occurs when cdc7ts cells grow at
36 °C.
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