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Figure 7.
Fig. 7. A: Hydrogen bondingpattern between residues 1 I5 nd 118 of nuclease A (Hynes & Fox, 1991) andthesurrounding
ordered water. Residues 115-1 8 adopt a type IVa &turn with a hydrogen bond between the carbonyl oxygen of Tyr 115 and
thebackboneamide of Asn 118. B: Hydrogen bonding pattern between residues 115 and 18 of 116D and thesurrounding
ordered water. The Asp 116 side chain replaces thebackbone of Tyr 115 n many of the hydrogen bonds observed in the wild-
typestructure. C: omparison of the residue 112-118 loopconformationsfromthe K116D variant (white) nd the 116G
nuclease variant (Hodel et al., 1992) (green). Oxygen atoms re colored red; nitrogen is colored blue.
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