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Figure 5.
The cartoon drawing for the mechanism how Hsp70/Hsp90
prepares Tom70/Tom71 for preprotein loading.a, Tom70/Tom71 may
exhibit two distinct states: the open and closed state. The
Tom70/Tom71 molecule is shown in blue. The N- and C-terminal
domains are labeled. The mitochondria outer membrane is shown in
orange. b, the interactions between the Hsp70/Hsp90 C-terminal
EEVD motif will lock the Tom70/Tom71 in the open state. The
Hsp70/Hsp90 is shown in gold. The binding between Hsp70/Hsp90
and Tom71 could increase the volume of the preprotein-binding
pocket. The complex formation might rotate the Tom71 C-terminal
domain ∼20° back toward the closed state and therefore
position the preprotein-binding pocket closer to the
Hsp70/Hsp90. The Hsp70/Hsp90 EEVD motif is shown as a red arrow.
The preprotein is shown as a green triangle. c, Hsp70/Hsp90 will
then load the preprotein into the enlarged preprotein-binding
pocket of Tom70/Tom71.
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