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Figure 5.
Figure 5 Schematic diagram of water arrangements in the active
sites of the reduced native and G81A mutant form of MDH-GOX2.
Hydrogen-bonding distances are in Å. (a) Native reduced
enzyme. The network of three water molecules (Wat169^WT,
Wat196^WT, Wat142^WT) and one sulfate ion are shown along with
their interactions with each other, with nearby side chains and
with two other waters, Wat110^WT and Wat375^WT. (b) G81A mutant
enzyme. The three waters (Wat282^GA, Wat283^GA and Wat284^GA)
displace the native sulfate ion and three-water network and take
up new positions. Wat54^GA is in the same position as Wat97^WT
of the native enzyme. R is a ribityl phosphate group and R' is a
Leu side chain.
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