|
Figure 5.
Figure 5. Analysis of the Hs-BUB1-Blinkin Interaction
(A) Yeast two-hybrid analysis of diverse Hs-BUB1 mutants. The
effect of the mutant P23G could not be established as it showed
self-activation.
(B) Projection of residues important for
binding Blinkin onto the protein surface (salmon color).
Residues whose mutation did not compromise the binding with
Blinkin are shown in blue.
(C) Mapping of Hs-BUB1 mutations
associated with cancer. Residues absent in the deletion mutant
Δ76–141 are shown in green and residues E36D, A130S, and
H151D in brown.
|
