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Figure 5.
Fig. 5. (a) Structural alignment of the active sites of
Nt.BspD6I (red), ss.BspD6I (green), FokI (marine) and BamHI
(grey). The segments 418–489 (Nt.BspD6I), 22–93 (ss.BspD6I),
425–488 (1FOK), 61–134 (2BAM) are depicted as cartoons,
omitting for each structure the loop between the first and the
second β-strand. Putative catalytic residues are shown as
sticks, the calcium ion sites A and B of 2BAM as spheres. (b)
Hydrogen bonding network in the catalytic center of the
Nt.BspD6I cleavage domain. The residues E418, D456, E469 and
E482 are essential for DNA cleavage activity.
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