Figure 5.
Measurement of FMN, riboflavin, or NADH binding by ITC
experiments. The trend of heat released by serial injections of
FMN (▪), riboflavin (▾), or NADH (•) into EmoB was
monitored. FMN showed the typical heat-releasing pattern.
Neither riboflavin nor NADH had any detectable heat-releasing
events upon injections. Solid lines represent the least-square
fits of the data using a single-site binding model.
The above figure is reprinted
from an Open Access publication published by the ASBMB:
J Biol Chem
(2008,
283,
28710-28720)
copyright 2008.
