Figure 5 - full size

 

Figure 5.
FIGURE 5. Comparison of GH95CBM51, GH98CBM51, and other CBM51 members. A, overlay of the GH95CBM51 (yellow) and GH98CBM51 (magenta) carbohydrate-binding sites. Relevant side chains involved in binding and ligands are shown in stick representation, and the metal atoms are shown as spheres. B, phylogenetic analysis of CBM51. The inset shows the complete analysis and indicates the subfamilies. Subfamilies CBM51a and CBM51b (circled in the inset) are expanded with detailed entries. The green star denotes the GH98CBM51 entry, and the blue star denotes the GH95CBM51 entry. C, alignment of subfamilies CBM51a (indicated by the green vertical line) and CBM51b (indicated by the blue vertical line). The entry numbering corresponds to that in B. GH98CBM51 and GH95CBM51 are indicated by stars as in B. The secondary structures for GH98CBM51 and GH95CBM51 are shown above and below the alignment, respectively. Yellow arrows denote β-strands, and the red cylinder represents an -helix. Residues involved in ligand binding by GH98CBM51 and GH95CBM51 are indicated above and below the alignment, respectively, by arrowheads.

The above figure is reprinted by permission from the ASBMB: J Biol Chem (2008, 283, 12604-12613) copyright 2008.