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Figure 5.
Figure 5. Electron Microscopic Analysis of Native TA2F[1]F[o]
(A) Electron micrograph of TA2F[1]F[o] negatively stained
with uranyl acetate. The scale bar represents 50 nm.
(B)
Class averages after multivariate statistical analysis and
classification of 1940 particles. At least 20% of particles were
rejected based on poor resolution, as well as 20% from each
class. Classes 1–8 contain 197, 250, 197, 194, 184, 261, 141,
and 129 particles, respectively. Classes 1–4 show a view with
the central stalk attached asymmetrically on the left side of
the c ring; classes 5–7 show a more centrally connected stalk
and a much narrower F[o] domain. Class 8 contains some poorly
aligned particles, of which some have the F[1] domain facing
down. Each frame represents 382 Å.
(C) Corresponding
views of the TA2F[1] structure rotated approximately 90°
relative to each other. The images were created using UCSF
Chimera (Pettersen et al., 2004).
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