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Figure 5.
FIGURE 5. Detail of the hITPK1 inositol phosphate binding
pocket. Identical projections for eITPK1 (A) and hITPK1 (B),
showing the reduction in inositol phosphate binding site volume
and ATP solvent accessibility for hITPK1. Ins(1,3,4)P[3] is
shown modeled into the hITPK1 based on structural alignment with
eITPK1 and exhibits a steric clash with His-162. A
solvent-accessible surface is shown for each molecule. The
position of the ATP  -phosphate is indicated
by " ". Metal ions are shown
as green spheres. Panel C is a stereodiagram showing structural
superposition of hITPK1 with eITPK1. hITPK1 is shown with blue
carbons atoms, with a bound sulfate shown in gold and red.
Residues capable of carrying a phosphate that are proximal to
the ATP -phosphate are shown in
yellow. eITPK1 in complex with Ins(1,3,4)P[3] is shown with
orange carbon atoms for protein, magenta carbons for the
inositol.
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