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Figure 5.
Figure 5. Mutagenesis Identifies Hot Spot Residues in the
Linker-Kinase Sandwich
(A) Two schematic views of ZAP-70,
rotated by 180° with respect to each other, depict the
location of residues in ZAP-70 that are most critical for
autoinhibition. These hot spot residues are indicated by yellow
stars.
(B) LAT phosphorylation by ZAP-70 mutants. The
Tyr315Ala/Tyr319Ala double mutant is referred to as “YYAA.”
Strongly activating alanine mutations at hot spots of the
linker-kinase sandwich are highlighted with yellow stars.
Control experiments in the presence of Lck are shown in Figure
S4.
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