Figure 5.
FIGURE 5. Buried polar interactions of position a
asparagine residues in the HY5 LZ domain. A, the electron
densities (2F[o] - F[c]) contoured at 1.5 of Asn^19 (left) and
Asn^33 (right) from each helix strand are shown. Hydrogen bonds
formed between the side chains of asparagines from each monomer
are indicated by dashed green lines. B, shown is the extensive
hydrogen bond network of Asn^33 in the HY5 LZ domain.
Interstrand and non-helical intrastrand hydrogen bonds are
formed in and around Asn^33. Hydrogen bonds are indicated by
dashed green lines. Distances are expressed in angstroms. Ribbon
diagrams of the residues from strands A and B are shown in
orange and purple, respectively. C, the hexad structure of
Asn^33, which consists of
Glu^32-Asn^33-Arg^37/Glu^32'-Asn^33'-Arg^37' (upper), is
contrasted with the corresponding regions that contain
Lys^18-Asn^19-Glu^23/Lys^18'-Asn^19'-Glu^23' (lower). The hexad
structure of Asn^33 is tightly packed, whereas that of Asn^19 is
loosely packed. Polar, negatively charged, and positively
charged residues are colored yellow, red, and blue,
respectively. The figure was prepared with the program
Swiss-PdbViewer (39).
The above figure is reprinted
by permission from the ASBMB:
J Biol Chem
(2007,
282,
12989-13002)
copyright 2007.
of Asn^19 (left) and
Asn^33 (right) from each helix strand are shown. Hydrogen bonds
formed between the side chains of asparagines from each monomer
are indicated by dashed green lines. B, shown is the extensive
hydrogen bond network of Asn^33 in the HY5 LZ domain.
Interstrand and non-helical intrastrand hydrogen bonds are
formed in and around Asn^33. Hydrogen bonds are indicated by
dashed green lines. Distances are expressed in angstroms. Ribbon
diagrams of the residues from strands A and B are shown in
orange and purple, respectively. C, the hexad structure of
Asn^33, which consists of
Glu^32-Asn^33-Arg^37/Glu^32'-Asn^33'-Arg^37' (upper), is
contrasted with the corresponding regions that contain
Lys^18-Asn^19-Glu^23/Lys^18'-Asn^19'-Glu^23' (lower). The hexad
structure of Asn^33 is tightly packed, whereas that of Asn^19 is
loosely packed. Polar, negatively charged, and positively
charged residues are colored yellow, red, and blue,
respectively. The figure was prepared with the program
Swiss-PdbViewer (39).