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Figure 5.
FIGURE 5. a, wild type GTB showing the position of Met-214
and Met-266 relative to bound UDP-Gal. b, M214T showing the
position of Thr-214 and Met-266 relative to bound UDP-Gal. The
predominant conformation of Met-266 (green) opens up the active
site. c, M214T modeled with UDP-GalNAc. The larger A-donor,
UDP-GalNAc, can be accommodated by the high occupancy
conformation of Met-266 (green) and not the low occupancy, wild
type conformation (yellow). Atoms are colored by element:
carbon, white; oxygen, red; nitrogen, blue; sulfur/phosphorous,
yellow. The modeled Gal and GalNAc of the donors are shown with
magenta bonds.
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