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Figure 5.
Figure 5. The ATPase site of the E151D mutant RadA in
stereo. Two MvRadA subunits are colored yellow and gray,
respectively. K^+, Mg^2+ and water molecules are colored purple,
red and green, respectively. (a) E151D RadA/AMP–PNP in a 0.5 M
KCl. (b) E151D RadA/AMP–PNP complex in 0.5 M NaCl. (c) E151D
RadA/ADP complex in 0.5 M KCl. Most residues in the L2 region
are ordered in (a), but similarly disordered in (b) and (c). In
addition to a nucleoside triphosphate and Mg^2+, K^+ appears to
be required for stabilizing the more ordered “active” L2
conformation.
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