|
Figure 5.
Fig. 5. (A) All active sites of the fungal FAS are oriented
toward the interior of the reaction chamber. The dome (lower
panel) is cut from the central wheel (upper panel) and flipped
open. Fitted domains are colored in light brown and unassigned
electron density is in gray. The trimeric connection at the
apices of the particle observed in the 8 Å–resolution
map is also shown in gray. Red cones indicate the entrances to
the hydrophobic clefts that lead to the active sites. (B) Set of
active sites in the reaction chamber with all enzymatic
activities required for the fatty acid synthesis cycle. The view
is into the reaction chamber, with one-third of the dome
removed. Distances between the central structural feature
(indicated by green spheres) and the active sites are indicated
with red lines. (C) Schematic path of ACP, shown as a gray
sphere, during substrate shuttling between the active sites.
|
