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Figure 5.
Figure 5. A close-up stereoview of the active site (the
S39A-H(R3HB)[3]M complex). Side-chains of catalytic residues and
residues forming the substrate-binding site in the crevice are
represented as ball-and-stick models. The amide nitrogen atoms
of Ser40, Cys250, and Asn302 are also shown. A model of the R3HB
trimer (designated as (R3HB)[3]) is also shown as a black
ball-and-stick model. A hydrogen bond between Nd1 atom of Trp307
and the carbonyl oxygen of an ester linkage adjacent to a
scissile linkage, and one between His155 and Asp121 are
indicated as green lines. The side-chain of Ser39 of the
wild-type protein (shown in red) is superimposed on Ala39 of the
mutant model, and a hydrogen bond between the Og atom of Ser39
and the amide group of Ser40 is tentatively indicated with a
green broken line.
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