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Figure 5.
Figure 5 A model of the interactions between a SeqA filament and
DNA. (A) A SeqA -N dimer and a pair of crystallographic SeqA -C
-DNA complexes are placed to share a common dyad axis. The
protein is shown as ribbon diagrams in dark (SeqA -N) and light
(SeqA -C) blue and the DNA depicted as stick models. (B) The
full-length SeqA dimer -DNA model is allowed to multimerize
according to the 4[3] screw axis of the SeqA -N filament. The
four-fold screw axis is perpendicular to the plane. Each SeqA -N
dimer and the SeqA -C pair bound to it are shown in dark and
light shades of a distinct color. Space between the N- and
C-terminal domains accounts for the flexible linker and avoids
contacts or clashes between neighboring SeqA -C molecules
related by the 4[3] screw axis. An orthogonal view placing the
SeqA -DNA superhelix in plane is shown on the right panel. No
artificial coordinates are introduced, and DNAs are left to be
discontinuous between adjacent SeqA dimers. Scale bars represent
100 Å.
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