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Figure 5.
FIG. 5. NS5A membrane anchor mutants. A, amino acid
sequences of NS5A membrane anchor mutants. The amino acid
conservation among different HCV isolates (20) is indicated by
an asterisk (*), a colon (:), and a dot (.) for fully conserved,
conserved, and similar residues, respectively. B and C,
space-filling representation of theoretical helices for mutants
8+A and 11+A. An Ala insertion (shown in magenta) twists the
helix by 110°. The C-terminal side of the helix is shown in
the same orientation as in Fig. 3B to highlight the distortion
of charged residues on the N-terminal side. Residues are colored
as in Fig. 3B. These models were constructed by using the NMR
average structure of NS5A[1-31] observed in 100 mM SDS as
template and SwissPdb Viewer program (www.expasy.ch/swissmod/).
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