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Figure 5.
Fig. 5. Catalytic mechanism. (a) A stereo view of
thermolysin residues [yellow, with side chain O atoms, red, and
side chain N atoms, blue; PDB 1TMN [PDB]
(28)] involved in catalysis is shown superimposed on the
homologous residues from BoNT/A (shown in cyan). P1 and P2 of
the cleavage product from pseudosubstrate are also shown with
the product carboxylate coordinated to the catalytic zinc.
BoNT/A LC Y365 (magenta) is shown in the conformation from
holotoxin. This figure was generated with MIDAS (36). (b) A
schematic illustration of the proposed catalytic mechanism of
250s loop hydrolysis in the BoNT/A homodimer is shown. This
figure was generated with ISIS DRAW.
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