|
Figure 5.
Figure 5. The active site and molecular basis of substrate
specificity. (a) Stereo view of TGT active site. The main
chains of TGT are grayish blue, the side chains of TGT are
orange and the RNA and 9dzG are green. The hetero-atoms are
colored individually, with nitrogen in blue, oxygen in red and
phosphate in magenta. The C9 of 9dzG is highlighted in black. A
water molecule is shown as a small ball in red and labeled 'W'.
Hydrogen bonds are indicated with dashed lines. The side chains
of Asp106 and Cys158, which block 9dzG from the top, are omitted
for clarity. (b,c) Recognition of U33 (b) and U35 (c) in RNA
substrate by TGT. The color schemes are the same as in a. The
structure of the phosphate group of A38 in c is omitted but
labeled for clarity.
|
