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Figure 5.
Figure 5. Stereo images of the least-squares fit between the
structures of the double mutant, bnLys27→Ala-bsAsp35→Ala,
and the pseudo wild-type structure. Pseudo wild-type barnase is
coloured white and barstar is coloured yellow; the double mutant
is coloured black. Additional water molecules observed in the
double mutant at the site of mutation are also shown. (a) The
site of the Asp35bs→Ala mutation in the AD complex. (b) The
site of the Lys27bn→Ala mutation in the AD complex. (c) The
site of the Lys27bn→Ala mutation in the BE complex. The
Figures were drawn with Bobscript [Esnouf 1997 and Kraulis
1991].
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