Figure 4.
(a) Schematic diagram of Nrd1. RE/RS, arginine-, serine- and
glutamate-rich region; P/Q, proline- and glutamine-rich region.
(b) Phenotypic analysis of nrd1 6–214
and nrd1 151–214
deletions. The NRD1 plasmid shuffling strain EJS101-9d was
transformed with pJC580, pRS415-Nrd1 6–214
or pRS415-Nrd1 151–214,
and the wild-type NRD1/URA3 plasmid (pRS316-NRD1) was shuffled
out on 5-fluoroorotic acid (5-FOA) medium at the indicated
temperatures. (c) Phenotypic analysis of nrd1 6–150
and nrd1 151–214
alleles integrated into the genome. (d) Nrd1 residues 151–214
are responsible for interaction with Nab3. Expression of the
wild-type (WT) and mutant TAP-tagged proteins Nrd1, Nrd1 6–214
and Nrd1 151–214
in extracts was monitored by immunoblotting for the Protein A
module of the TAP tag (below, -TAP).
Nrd1 protein complexes were purified using IgG resin, and
association with Nab3 was analyzed with anti-Nab3 antibody
(above). Note that the Protein A module on Nrd1 reacts with the
secondary antibody. (e) The Nab3 and CTD binding regions of Nrd1
contribute to its interaction with Pol II in vivo.
The above figure is reprinted
from an Open Access publication published by Macmillan Publishers Ltd:
Nat Struct Biol
(2008,
15,
795-804)
copyright 2008.
6–214
and nrd1 
6–214
or pRS415-Nrd1 
-TAP).
Nrd1 protein complexes were purified using IgG resin, and
association with Nab3 was analyzed with anti-Nab3 antibody
(above). Note that the Protein A module on Nrd1 reacts with the
secondary antibody. (e) The Nab3 and CTD binding regions of Nrd1
contribute to its interaction with Pol II in vivo.