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Figure 4.
Fig. 4. Crystal structure of ZmCKO1 inactivated by allenic
cytokinins. (a) Overall surface of the enzyme. β-Strands are
indicated in cyan and α-helices in light blue. FAD cofactor
(yellow), bound HA-1 inhibitor (green) and N-acetyl-glucosamines
(orange) are shown in space-filling CPK representation with
nitrogen atoms in blue and oxygen atoms in red. The five
glycosylated Asn sites are numbered. (b and c) Binding of HA-1
(carbon atoms in green) and HA-8 (carbon atoms in black) in
their 2F[o] − F[c] maps; contoured at 2σ. The new covalent
bond between the C3 atom of the inhibitor side chain and the C4a
atom of FAD is shown in brown. (d) Hydrogen-bonding interactions
of the inhibitor HA-1 at the active site of ZmCKO1. (e) Detail
of the covalent bond between HA-1 inhibitor and FAD cofactor.
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