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Figure 4.
Fig. 4. Crystal structures of the complex of Fab MOR03268:TSC
in two crystal forms. (a) Overall view of the 1:1 complex in
crystal form 1. The Fab molecule is shown in secondary structure
and surface representation, with the heavy chain depicted in
blue and the light chain in orange. TSC (stick representation)
is bound within a deep surface cavity in the antigen-binding
site located at the interface between the two N-terminal
variable immunoglobulin domains (V[L] and V[H]). C[H] and C[L]
refer to the constant immunoglobulin domains of the heavy and
light chain. (b) Stereo representation showing the detailed
binding mode of TSC in form 1. Both the light chain (orange) and
the heavy chain (blue) contribute three loops, the
complementarity determining regions (CDRs). (c) Overall view of
the 2:1 complex in crystal form 2. TSC is bound in the interface
between two Fab molecules, resulting in a TSC-induced Fab dimer
with a very elongated molecular shape. The zoom on the right
side shows a cross section through the Fab dimer interface,
illustrating that the bound TSC molecule site is fully shielded
from the solvent.
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