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Figure 4.
Figure 4. Electron density maps of N2C/D282C rhodopsin. (a)
Retinal omit electron density map (0.75 σ) obtained after
molecular replacement and density modification. The retinal
(red) was not included but appears clearly in the electron
density. (b) Disulfide bond and N15 glycosylation. The
σA-weighted 2F[o] – F[c] with electron density contoured at
0.9σ. The disulfide bond between the mutated residues C2 and
C282 is well resolved, as is the first residue of the
N-glycosylation linked to N15. No density is visible adjacent to
the mutated residue 2, which would have been glycosylated in
wild-type rhodopsin.
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