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Figure 4.
Monolayer penetration of the Bem1p PX and OSBP and FAPP1 PH
domains into various phospholipids.A, Δπ was measured as a
function of π[0] for wild-type Bem1p-PX with POPC/POPE (80:20)
(○), POPC/POPE/PtdIns(4)P (77:20:3) (•),
POPC/POPE/PtdIns(3)P (77:20:3) (▪), POPC/POPE/PtdIns(5)P
(77:20:3) (▴), and POPC/POPE/POPS (60:20:20) (□) monolayers.
B, Bem1p (○), OSBP (□), and FAPP1 (▵) were allowed
interact with the POPC/POPE (80:20) monolayer, or Bem1p (•),
OSBP (▪), and FAPP1 (▴) were added to the
POPC/POPE/PtdIns(4)P (77:20:3) monolayer. The subphase consisted
of 10 mm HEPES (pH 7.4) containing 0.16 m KCl. n = 2.
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