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Figure 5.
Figure 5. Superposition of chagasin with the active site of
cruzipain. (a) A ribbon stereo diagram of the superposition of
the chagasin–cathepsin L complex (colored as in Figure 3(a))
with cruzipain (burgundy), after least-squares fit of the C^α
traces of the enzymes. The view is in the standard orientation
along the active site cleft of the enzymes. (b) A detailed view
of the interactions showing the central part of the chagasin
inhibitory wedge (with the L4 loop light yellow and the L2 loop
orange) and the complementary fragment of the
L-domain of cruzipain (burgundy). The side-chains of
residues crucial for the interactions are shown in
ball-and-stick representation. The synthetic inhibitor P10
([(1-(3-hydroxy-2-oxo-1-phenethyl-propylcarbamoyl)2-phenyl-ethyl]-carbamic
acid pyridin-4-ylmethyl ester), from the complex with cruzipain
(PDB code 1ME3) is shown in green.
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