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Figure 4.
FIGURE 4. Superposition of G336V,G337V + serine with G336V
tetramers. Left, the figure depicts a superposition of
serine-bound G336V,G337V double mutant onto a G336V tetramer.
The serine-bound G336V,G337V tetramer is generated using the
symmetry-related molecules. Subunit A and C are colored orange,
and subunits B and D are colored pink. In the G336V structure,
the subunits A and C are colored with shades of green, and
subunits B and D are colored with shades of blue. Subunits A and
C of G336V,G337V + serine superimpose well on the respective A
and C subunits of the G336V serine-free enzyme. In subunits B
and D, there is rotation of the nucleotide binding domain of
10-12°. In both the tetramers, ligands are not shown for the
purpose of clarity. Right, detailed view of the hydrogen bond
interaction at Asn^190 of subunits A and C of G336V,G337V +
serine. In subunits B and D (pink), the Asn^190 residues are
separated by too great a distance to form hydrogen bond contacts.
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