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Figure 4.
Figure 4. Peptide Binding to Mutant PDZ Domains
Stopped-flow data on representative mutants of PDZ domains.
Mutants were classified into three categories: those primarily
altering the association rate constant (A), the dissociation
rate constant (B), or both (C). See Table 2 for rate constants.
(D) Effect of mutagenesis on binding. PDZ2 is shown in ribbon
representation (gray); residues affected by mutagenesis in
space-filling representation. Blue-colored residues primarily
alter the association rate constant, salmon-colored residues
affect the dissociation rate constant, whereas magenta-colored
residues affect both rate constants. The P[−3]-P[0] residues
of the APC-peptide are also shown in red by using stick
representation.
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