Figure 4.
Fig. 4. Coenzyme binding pocket. (A) Molecular surface of the
glmS ribozyme (orientation corresponds to Fig. 1B). Nucleotides
>90% conserved are colored green. Atomic spheres are shown for
Glc6P and the Mg^2+ ion that coordinates its phosphate. (B)
Expanded view of the Glc6P binding pocket. (C) Portion of the
Glc6P-bound, 2'-deoxy A^(–1) precleavage structure
superimposed on the residual simulated-annealing omit |F[o]| –
|F[c]| electron density map contoured at 2.5 [orientation is
rotated slightly from (A)] and a stick figure of the bound
Glc6P. Hydrogen bonding interactions involving Glc6P and two
active site water molecules (red spheres) are shown. The
position of a modeled 2'-OH of A^(–1) is shown to indicate its
proximity to one of the two buried waters.
The above figure is reprinted
by permission from the AAAs:
Science
(2006,
313,
1752-1756)
copyright 2006.
[orientation is
rotated slightly from (A)] and a stick figure of the bound
Glc6P. Hydrogen bonding interactions involving Glc6P and two
active site water molecules (red spheres) are shown. The
position of a modeled 2'-OH of A^(–1) is shown to indicate its
proximity to one of the two buried waters.