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Figure 4.
Figure 4. Stereo views of the WT M.HhaI and R165A M.HhaI bound
to cognate DNA, and the WT M.HhaI bound to DNA with constrained
sugar analog substitution.^25 (a)1 The active site of the WT
M.HhaI is shown with Cys81, Glu119, Val121, Arg163, Arg165, and
AdoHcy, which have contacts (dotted red lines) to the flipped
out cytosine base (3MHT.pdb). (a)2 The flipped out cytosine is
positioned in the active site pocket in the WT M.HhaI protein.
(b)1 The active site of the R165A M.HhaI is shown with Cys81,
Glu119, Asn120, Val121, Arg163, Glu164, and Ala165, which have
contacts to the flipped out cytosine base (dotted red lines).
(b)2 The flipped out cytosine base is positioned in the active
site pocket in the R165A M.HhaI protein. (c) The flipped out
South-constrained abasic carbocyclic sugar is rotated in the
active site pocket in the WT M.HhaI protein (1SKM.pdb).The
distance unit is Å and the heteroatom colors are: red,
oxygen; blue, nitrogen; orange, sulfur; hot pink, phosphorus.
Figure 4. Stereo views of the WT M.HhaI and R165A M.HhaI bound
to cognate DNA, and the WT M.HhaI bound to DNA with constrained
sugar analog substitution.[3]^25 (a)1 The active site of the WT
M.HhaI is shown with Cys81, Glu119, Val121, Arg163, Arg165, and
AdoHcy, which have contacts (dotted red lines) to the flipped
out cytosine base (3MHT.pdb). (a)2 The flipped out cytosine is
positioned in the active site pocket in the WT M.HhaI protein.
(b)1 The active site of the R165A M.HhaI is shown with Cys81,
Glu119, Asn120, Val121, Arg163, Glu164, and Ala165, which have
contacts to the flipped out cytosine base (dotted red lines).
(b)2 The flipped out cytosine base is positioned in the active
site pocket in the R165A M.HhaI protein. (c) The flipped out
South-constrained abasic carbocyclic sugar is rotated in the
active site pocket in the WT M.HhaI protein (1SKM.pdb).The
distance unit is Å and the heteroatom colors are: red,
oxygen; blue, nitrogen; orange, sulfur; hot pink, phosphorus.
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