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Figure 4.
Figure 4: Bioluminescence colour of wild-type and three mutant
forms (I288V, I288A and S286N) of Lcr luciferases. a,
Photographs of bioluminescence by a luciferase-catalysed
reaction. The reaction condition was 0.1 mM luciferin, 2 mM ATP,
6 mM MgSO[4], 25 mM HEPES (pH 7.8) and 0.1 mg ml^-1 luciferase
in 0.5 ml solution. b, Emission spectra of the four types of
Luciola cruciata luciferases.
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