|
Figure 4.
Thermodynamic cycles for stepwise mutation of wild-type
streptavidin to the S45A/D128A double mutant, showing
[Delta]G[298], [Delta]H[298], and T[Delta]S[298]. The cycle
starts with wild type in the upper left corner and proceeds to
the single mutants, with the thermodynamic perturbation shown
next to the arrows. The cycle continues to the double mutant,
with the difference in thermodynamic parameters between each
single mutant and the double mutant shown next to those arrows.
The units on the quantities in the figure are kcal/mol.
|
