|
Figure 4.
Figure 4. The Zn2+ binding site of EZ-MBP-HA. (a) The
structure of the Zn2+-binding site of EZ-MBP-HA. The refined
structure of the Zn2+-bound protein was used to calculate a
2F[O] -F[C] electron density map (gold, contoured at 1s). To
positively identify bound Zn ions, data were collected at the
absorption peak for Zn2+(1.2824 Å) and used to calculate
an anomalous difference map (red, contoured at 3 s). (b) The
structure of EZ-MBP-HA in the absence of Zn2+. The refined
structure of Zn2+-free EZ-MBP-HA was used to calculate a
2F[O]-F[C] electron density map (contoured at 1s). (c) A
superposition of the Zn2+-free (yellow) and Zn2+-bound (grey)
structures of EZ-MBP-HA, showing the movement of H63 and H66 to
coordinate Zn2+ atoms. Figures were prepared using Spock,26
Raster3D,27 and SwissPDBViewer.28
|
