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Figure 4.
Figure 4. Hypothetical interaction between RNA and the
hexadecameric nsp7–nsp8 complex. (a) The electrostatic
potential surface of the hexadecamer modeled with (right) and
without (left) duplex RNA in the positive channel. Blue,
positive charge (+10 k[B]T); red, negative charge (-10 k[B]T).
(b) Model of the hexadecameric nsp7–nsp8 supercomplex with
hypothetical duplex RNA. Left, top view, showing the channel's
proper dimensions to accommodate dsRNA. Right, side view,
showing a possible mode of interaction where the four nsp8II NH3
helices insert into the dsRNA grooves. (c,d) Results of EMSAs of
nsp7, nsp8 and their mutants. Each lane contains 75 pmol dsRNA
(c) or dsDNA (d). Lanes 1–7 contain 650 pmol of each protein;
lanes 8–13 contain 90 pmol of hexadecamer.
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