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Figure 4.
Figure 4. Alignment of free and liganded structure and
detailed comparison of CDR-H3 conformation. (A) Least squares
superposition of V[L] in the unliganded FITC-E2 scFv (blue) and
the FITC-E2-hapten complex (red), indicating the difference in
the relative orientation of V[L] and V[H] in the two structures.
The axis of a 3° rotation relating the V[H] orientations in the
freeand the liganded scFv, located in the plain of thefigure, is
indicated bya small arrow. (B) CDR-H3 conformation in the
unliganded scFv (blue) and the liganded scFv (red). Equivalent
atoms are related by green arrows.
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