|
Figure 4.
Figure 4. Molecular recognition of RNA by TIS11d and hydrogen
bonding between TIS11d and RNA. (a) Molecular contact surface
of a representative TIS11d TZF structure generated in GRASP51
showing surface topology. Green denotes convex surfaces. The
locations of the (R/K)YKTEL motifs that form the U6 and U2
binding pockets are indicated. (b) Stereo view showing
hydrogen-bonding interactions between finger 1 and the 3' UAUU
subsite. The protein backbone is light blue, the RNA backbone
and bases pale yellow, and the intercalating aromatic side
chains green. Each of the intermolecular hydrogen bonds is
designated with a number and identified by a red line and
colored atoms (red, oxygen; white, hydrogen; blue, nitrogen).
(c) Schematic figure summarizing hydrogen-bonding interactions
(dotted lines) with each of the bases in both subsites of the
ARE. The hydrogen bond numbers for finger 1 in part b are shown.
Panels a and b were generated using MolMol50.
|
