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Figure 6.
Figure 6. Mutational Analyses of CBP Bromodomain Binding to
p53(A) Effect of point mutation of CBP bromodomain residues on
p53 peptide binding. Western blot with GST antibody shows
GST-CBP bromodomain binding to the biotinylated p53 AcK382
peptide bound to streptavidin-agarose beads (top). Relatively
equal amount of proteins was used in each binding experiment
(bottom). Mutants highlighted in red exhibited markedly reduced
binding to the p53 peptide. Mutational effects on the
protein/peptide binding were quantitated.(B) Mutational analysis
of p53 peptide residues, assessed in a competition assay as
described in Experimental Procedures. Mutant p53 peptides that
showed a major reduction in binding to the bromodomain are
indicated in red.
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