|
Figure 4.
Figure 4. FAD binding in BenC. (a) The FAD-binding site is
situated between the FAD-binding domain, in yellow, and the
NADH-binding domain, in blue. The methyl groups of the
isoalloxazine ring are pointing towards the [2Fe-2S] center in
the ferredoxin-like domain. The FAD is represented inside a F[o]
-F[c] map, colored in green, contoured at 4×rms. The
picture was made with the program BOBSCRIPT
(http://www.strubi.ox.ac.uk/bobscript/). (b) A close-up of the
FAD molecule binding to the NADH and FAD binding domains of
BenC. There are five water molecules contributing to the
extensive hydrogen bonding holding the FAD in position. The
stacking interaction between Phe335 and the isoalloxazine ring
of the FAD can be seen at the bottom right of the Figure.
|
