Figure 4.
Fig. 4. PEP-binding site. A, polar contacts of PEP inside
the active site. The C- trace and
side chains of the Cd^2+ enzyme are shown with salmon and white
bonds, respectively. Cd^2+ coordination is shown as transparent
bonds. PEP is shown as ball-and-sticks with gold bonds; the si
side of PEP is pointing up and to the left, and the re side is
pointing down and to the right. Blue, nitrogen; red, oxygen;
yellow, sulfur; pale blue, phosphorus; orange, carbon. B,
details of PEP geometry inside the active site. PEP is shown
with its si side pointing up. The angle between the plane
defined by C-2, C-1, and C-3 (blue triangle) and the plane
defined by C-1, C-3, and O-2 (yellow triangle) is ~12°,
indicating that the geometry of C-2 is intermediate between
trigonal and tetrahedral. WAT, water.
The above figure is reprinted
by permission from the ASBMB:
J Biol Chem
(2001,
276,
8393-8402)
copyright 2001.
trace and
side chains of the Cd^2+ enzyme are shown with salmon and white
bonds, respectively. Cd^2+ coordination is shown as transparent
bonds. PEP is shown as ball-and-sticks with gold bonds; the si
side of PEP is pointing up and to the left, and the re side is
pointing down and to the right. Blue, nitrogen; red, oxygen;
yellow, sulfur; pale blue, phosphorus; orange, carbon. B,
details of PEP geometry inside the active site. PEP is shown
with its si side pointing up. The angle between the plane
defined by C-2, C-1, and C-3 (blue triangle) and the plane
defined by C-1, C-3, and O-2 (yellow triangle) is ~12°,
indicating that the geometry of C-2 is intermediate between
trigonal and tetrahedral. WAT, water.