|
Figure 4.
Fig. 4. Binding mode of acarbose to amylomaltase. (A)
Acarbose bound to the active site cleft and (B) acarbose near
Tyr54. Oxygen atoms are shaded grey and nitrogen atoms black.
Hydrogen bonding interactions are shown by dashed lines and the
interatomic distance is given. (A) and (B) were prepared using
LIGPLOT [55]. (C) Superposition of selected active site residues
of the amylomaltase–acarbose complex (carbon atoms colored
yellow) and a mutant Bacillus circulans CGTase bound to a
maltononaose substrate (PDB entry 1cxk [45]). Only the glucan
residues bound to subsites -1 and +1 are shown (programs
MOLSCRIPT [52] and RASTER3D [53]).
|
